How do you know how much material to spot in each lane of the TLC plate? What wo
ID: 1001948 • Letter: H
Question
How do you know how much material to spot in each lane of the TLC plate? What would happen if you spotted too much or too little sample? 4. What would occur if your developing solvent was above the line where your samples were spotted? 5. What would happen if you forgot to put a solvent wick in your developing chamber when performing a TLC run? 6. Visualizing spots once the plate is run is crucial in determining the components in the mixture. The above TLC was stained with vanillin in sulfuric acid to visualize the compounds in each essential oil. In our lab, UV will visualize compounds with conjugated double bonds and iodine will stain double bond-containing compounds and some oxygen containing functional groups. Which visualization method would you perform on each of the five molecules above? 7. What arc some possible reasons that we did not employ TLC analysis on the Group Project? What advantages does gas chromatography have over TLC?Explanation / Answer
3. If we spot too much materil then then it will not move up properly and it get spresed all over plate easily. The minimum amount should be like maximum one dropof diluted sample with help of capillary tube.
4. if develpooing plate solvent is above the line then all spot will disolve away in solvent.
5. There will not be any effect on TLC plate.we just need to dry once morebefore again perforimg the TLC.
6. We will perform UV-light for conjugate double band and stain diuble bond. we will use iodine for oxygen cintaing functional group.
7. Disdvatage : plate height is limited so seperation only till limited height and chances of affected by humdity and high temp. Gas chromtaography show high resolution, good qunatification of resul and choice of stationary phase and column with it'sreusability.
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