in the process of revising a lab protocol for the ... Your question has been ans
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in the process of revising a lab protocol for the ... Your question has been answered! Rate it below. Let us know if you got a helpful answer. Question In the process of revising a lab protocol for the study of integral membrane proteins, the procedure calls for .1% lauryl maltoside to form micelles to mimic the native behavior. However, you only have Triton X-100 available for conducting your experiment. What is the minimal amount, in g, of Triton X-100 you would need to dissolve per mL of your protein solution in order to achieve the desired effect?
non-ionic detergent such as Tritin X-100 non-denaturing, they break lipid-lipid interaction and lipid-protein interaction rather than protein-protein interaction. The proteins reconstituted into liposomes and destabilized by trition X-100. 40 micro grams of trition X-100 is applicable to a small amount of protein in micro grams provided and easily determined. How is the 40 micro grams found??
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in the process of revising a lab protocol for the ... Your question has been answered!
The procedure is called cell lysis and protein extraction. In this procedure by effect of the surfactant the cell which is made of a double layer of lypids breaks itself freeing the protein which get trapped in miscelles.
What is the minimal amount, in g, of Triton X-100 you would need to dissolve per mL of your protein solution in order to achieve the desired effect?
The minimal amount of surfactant or detergent needed to break the cells membrane and to form the miscelles is called CMC or crytical miscellar concentration. For Triton X-100 that amount is a solution of 0.24mM
Mass in g per mL of Triton X 100 needed = Concentration x volume x Molar weight of Triton X100 =
0.24 x10-3M x 1x10-3L x 625 g = 1.5x10-4 g is the amount of triton you need to dissolve to obtain miscelles per mL of protein solution
The proteins reconstituted into liposomes and destabilized by trition X-100. 40 micro grams of trition X-100 is applicable to a small amount of protein in micro grams provided and easily determined. How is the 40 micro grams found??
When working with membrane proteins, a general rule of thumb is to work at a detergent concentration of at least 2X CMC and at a detergent:protein weight-to-weight ratio of at least 4:1
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