A polypeptide is cleaved into peptides by treatment with trypsin and cyanogen br

Question

A polypeptide is cleaved into peptides by treatment with trypsin and cyanogen bromide, and the peptides are purified and sequenced. The sequences of the peptides are shown below.

trypsin peptides cyanogen bromide peptides

T-1 GASMALIK                                 C-1 EGAAYHDFEPIDPRGASM                  

T-2 EGAAYHDFEPIDPR                  C-2 TKDCVHSD

T-3 DCVHSD                                     C-3 ALIKYLIACGPM

Based on sequences of the overlapping peptides generated by treatment with trypsin and cyanogen bromide (shown above), which of the peptides could represent the N-terminus of the polypeptide?

Explanation / Answer

The N-terminus also known as the amino-terminus,NH2-terminus, refers to the start of a protein or polypeptide terminated by an amino acid with a free amine group (-NH2). By convention, peptide sequences are written N-terminus toC-terminus

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