Please answer all of the questions with explanation Thank you. You have isolated

Question

Please answer all of the questions with explanation

Thank you.

You have isolated a small region of double stranded DNA from E. coli below: 123123123123123123123123123123123123123123123 5'-ATCAATCCAGTCAGTACGTTACGTTGCTGAGTTTACGTTGATTCT-3' 3' -TAGTTAGGTCAGTCATGCAATGCAACGACTCAAATGCAACTAAGA-5' 321321321321321321321321321321321321321321321 a. The numbers on each strand indicate six difference reading frames for this stretch of DNA. Which reading frame on the top or bottom strand will most likely code for a long protein? b. What problem can you see with this approach in eukaryotes?

Explanation / Answer

a) TOP STRAND

if we will start from 1 then protein sequence will be-

INPVSTLRC&

if we will start from 2 then protein sequence will be-

SIQSVRYVAEFTLI

if we will start from 3 then protein sequence will be-

QSSQYVTLLSLR&

BOTTOM STRAND in all three possible situations we get termination in between. we did not get full-length protein.

b) the main problem with this approach in eukaryotes is the presence of introns in the gene.

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