UESTION 9 You identify an RFLP in mice by digesting genomic DNA with Hind III en
ID: 100650 • Letter: U
Question
UESTION 9
You identify an RFLP in mice by digesting genomic DNA with HindIII enzyme and radiolabeling a piece of probe DNA. Southern blot analysis shows that the probe detects a 2-kb fragment in one strain of mice and a 4-kb fragment in another strain of mice. The two mice strains are crossed to produce an F1 generation. Two F1siblings are mated to produce a dozen F2 progeny. You isolate genomic DNA from several F1 and F2 individuals, digest with HindIII, and perform a Southern blot with the same probe.
How many bands would be seen in the Southern blot of an F1 individual?
two
four
one or two
one
three
Q2-
Ampicillin resistance is to ampR as _____ is to lacZ.
gancyclovir
cleavage of X-gal
penicillin resistance
G418
Bt toxin
Q3-
A.two
B.four
C.one or two
D.one
E.three
Explanation / Answer
1.a.As probe detects a 2-kb fragment in one strain of mice and a 4-kb fragment in another strain of mice.Thus 2 bands would be seen in the Southern blot of an F1 individual.
2.b. The antibiotic & Xgal markers provide a selection scheme for recombinant bacterial colonies, as follows. First, only bacterial colonies that contain a plasmid-borne antibiotic-resistance gene can grow at all. Second, of those that grow, only those whose plasmids carry a functional LacZ in the polylinker can metabolize Xgal.
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