1- A cDNA encoding a protein that is specific for a particular strain (strain Q)
ID: 100656 • Letter: 1
Question
1- A cDNA encoding a protein that is specific for a particular strain (strain Q) of bacteria has been cloned. How would you determine whether an infection contains this particular bacterial strain?
2- Which of the following remains a problem of gene therapy?
We have yet to develop a vector for delivering the gene.
We do not have the ability to use somatic gene therapy at this time.
To date, there has not been a case of gene therapy curing a disease.
We can only alter germ-line cells at this time.
Patients mount immune responses to the transferred gene and vectors.
2-
origin of replication.
selectable markers.
unique restriction enzyme sites.
Ti plasmid.
3-
We have yet to develop a vector for delivering the gene.
B.We do not have the ability to use somatic gene therapy at this time.
C.To date, there has not been a case of gene therapy curing a disease.
D.We can only alter germ-line cells at this time.
E.Patients mount immune responses to the transferred gene and vectors.
2-
origin of replication.
B.selectable markers.
C.unique restriction enzyme sites.
D.Ti plasmid.
3-
Explanation / Answer
2.a. Introducing changes into the wrong cells Targeting a gene to the correct cells is crucial to the success of any gene therapy treatment. Just as important, though, is making sure that the gene is not incorporated into the wrong cells. Delivering a gene to the wrong tissue would be inefficient, and it could cause health problems for the patient.For example, improper targeting could incorporate the therapeutic gene into a patient's germline, or reproductive cells, which ultimately produce sperm and eggs. Should this happen, the patient would pass the introduced gene to his or her children. The consequences would vary, depending on the gene.
3.d. The vector contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme that cuts the DNA. A cloning site,origin ,selectable requirements are other such requirements.
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