5. A number of agents have been demonstrated to cause protein hydrolysis or prot
ID: 1008017 • Letter: 5
Question
5. A number of agents have been demonstrated to cause protein hydrolysis or protein denaturation. Match the reagent or condition with the type of noncovalent interactions it would d Leters may be used more than once or not at all. in whether this is an example of denaturation or protein hydrolysis. (9) a. peptide bonds b. disulfides C. hydrophobic interactions d) ionic interactions e. H-bonding with surface Hao Agent or condition T of interaction disrupted Hydrolysis or Denaturation Type Temperature elevated above protein optimal temperature (Heat alone). Strong acid, 6 M HCI at 100 C for 24-48 hours. increased pH alone, above the pH optimum for the protein. Chloroform nonpolar solvent High salt concentration Polar organic solvents such as alcohols Detergents High concentrations of reducing agents such as mercaptoethanol Chaotropic reagents such as urea 6. Consider the following structure (10 pts) Put a on each of the chiral carbons on this structure. CH2OH C o ii) carefully draw the two anomeric forms of the predominant form(s) of the HCOH monosaccharide when dissolved in water. HOCH HCOH CH2OH iii) umber the carbons on this structure. Identify the anomeric carbon on each of these structures. iv) Circle the hemiketal on the structure you have drawn.Explanation / Answer
Q.5: Temperature raised above protein optimal temperature ---> breaks H - bonding with surface H2O (denaturation)
Strong acid, 6M HCl at 100 DegC for 24-48 hours ---- > Breaks peptide bond (through Hydrolysis)
Increased pH alone, abve the pH optimum for the protein ---- > breaks H - bonding with surface H2O (denaturation)
Chloroform non-polar solvent ---- > Breaks hydrophobic interaction(denaturation)
High salt concentration ------ > Disrupts ionic interactions(denaturation)
Polar organic solvents such as alcohols --- > breaks H - bonding with surface H2O (denaturation)
Detergents -----> Hydrophobic interaction (denaturation)
High concentration of reducing agents such as mercaptoethanol ---- > Breaks disulfide bonds(denaturation)
Chaotropic agents such as ures ---- > breaks H - bonding with surface H2O (denaturation)
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