If the restriction end nuclease PSTN it used to cut the foreign ONA and the plas

Question

If the restriction end nuclease PSTN it used to cut the foreign ONA and the plasmid pBR322 In a ONA cloning process, which colonics contain recombinant plasmid after transformation Colonies can survive in media of both amoxicillin and tetracycline Colonies cannot survive in media of either amoxicillin or tetracycline Colonics can survive in media of amoxicillin bot not in tetra Caine Colonies can survive in media of tetracycline but not m amoxicillin None of above Which of the following can cause mutation of your ONA X-ray radiation UV light Alkylating agent Spontaneous deamination of ammo group in a nucleic base All above

Explanation / Answer

Mutation is the change in the base in DNA trands. UV light is a cause of DNA mutatuion. This highly affects the basea those are connected via 2 H bonds. UV light can break those bonds easily

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