Question:Suppose a sample from a bottle of mouthwash gives an absorbance reading

Question

Question:Suppose a sample from a bottle of mouthwash gives an absorbance reading of 0.221. What is the concentration (M) the blue dye in the mouth wash.

Graphing info:

When Beer's Law is used in combination with a spectrophotometer, quantitative data is obtained. That data can be graphed and a linear regression will produce an equation that can be used to predict an unknown concentration. Consider the experiment below.

The absorbance data for a standard solutions of blue dye is given below. Construct a Beer's law calibration curve in which Absorbance is plotted on the y-axis versus concentration of Blue Dye #1 on the horizontal axis.

must show: data points, best fit, linear equ, linear regression, title, labels, units.

heres my graph below, I dont think I did it right, and I have no idea how to figure out the question above.

Standard Concentration (M) Absorbance A 1.00E-05 0.100 B 1.50E-05 0.157 C 2.00E-05 0.210 D 2.50E-05 0.280

Explanation / Answer

You plotted it right

The graph will be

The equation is y = 11073x - 0.005

y = absrobance

x = concentration

slope = 11073

intercept = -0.005

So we can get the concentration if we know the absrobance

Given : ABsrobance = 0.221

Let us put it in the equation

y = 11073x - 0.005

0.221 = y = 11073x - 0.005

0.221 +0.005 = 11073x

x = concentration = 2.041 X 10^-5

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