Why is it important to inoculate only a single tube per organism when performing

Question

Why is it important to inoculate only a single tube per organism when performing the MRVP tests? ___________________ During lab, your lab partner bumps into you, jostling your arm while you are inoculating your SIM tube. Is it still possible to reliably evaluate the sulfide, indole, and motility results from this tube? Which reactions will still be accurate? ___________________ The case study describes a cluster of food poisoning incidences. If the causative agent is Salmonella spp., then give your prediction of the IMViC test results. ___________________

Explanation / Answer

3) MRVP test differentiates organisms that follow mixed acid fermenters and butylene glycol pathways. It is important to inoculate only a single tube per organism while performing the MRVP tests because the results of the test can be compared reliably.

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