The media used for the mixed acid and neutral end product termination test is ca

Question

The media used for the mixed acid and neutral end product termination test is called a) MR-VP b) Nutrient broth c) Nutrient agar d) Urea agar To detect the presence of mixed acids during fermentation, 5 drops of ______ should be added to the media and a red coloration should be observed. a) Iron chloride b) Kovac's reagent c) Methyl red d) Hydrogen peroxide The pH indicator found in the specific sugar fermentation tubes is _____ When organism ferments that particular sugar, the media turns from red to ____ a) Litmus paper: orange b) Benedict's reagent: red c) Phenol red: yellow d) Kovac's reagent: purple Usually when an organism such as E.coli is positive for mixed acid fermentation, it a) Neutral end product fermentation b) Citrate fermentation c) Oxidase test d) Nitrate reduction

Explanation / Answer

1 ans-A E. coli.

Acid production on both (open and covered) tubes aerobiabbly and anerobically and color change from green to yellow,organism is fermentative

2 ans-A- MR-VP

Methyl Red & Vogues-Proskauer Test- MR used for the mixed acid fermentation of glucose and VP test used for the digestion of glucose to acetylmethylcarbinol is a neutral end product.

3 ans-Methyl red test

because of a pH at or below 4.4 from the fermentation of glucose, media becomes red after addition of MR.

4ANS-C -phenol red, yellow

Phenol red is a pH indicator which turns yellow, medium contains peptone, phenol red and the carbohydrate to be tested. red- neutral/alkaline, yellow-acid

5 ans- A-neutral end product fermentation

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