Given the following data for an enzyme-catalyzed reaction known to follow michae

Question

Given the following data for an enzyme-catalyzed reaction known to follow michaelis-menten kinetics,estimate the Km and Vmax. Show how you could use alineweaver-Burke plot to determine these constants. Vo (umol/min) Substrate added(mmol/L) 217 0.8 325 2 433 4 488 6 647 1000
Given the following data for an enzyme-catalyzed reaction known to follow michaelis-menten kinetics,estimate the Km and Vmax. Show how you could use alineweaver-Burke plot to determine these constants. Vo (umol/min) Substrate added(mmol/L) 217 0.8 325 2 433 4 488 6 647 1000
Vo (umol/min) Substrate added(mmol/L) 217 0.8 325 2 433 4 488 6 647 1000

Explanation / Answer

1/V

0.001546

1/S

1.25
0.5
0.25

0.166666667
0.001

From the plot where x = 1/S and y =1/V

intercept = 1/Vmax = 1/0.00154 = 649.3

slope = Km / Vmax = 0.041 = Km / 649.3 = 26.62 = Km

0.004608 0.003077 0.002309 0.002049

0.001546

1/S

1.25
0.5
0.25

0.166666667
0.001

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