Draw the structures of intermediate and the product of the reaction (that occurs

Question

Draw the structures of intermediate and the product of the reaction (that occurs in the TCA cycle) shown below. Indicate which step involves addition of water and which involves removal of water. Name the intermediate, the product and the enzyme that carries out this reaction in the TCA cycle. Define isozymes. Name two out of three isozymes (that function in gluconeogenesis) we discussed in class, mention the cellular location of the reaction they catalyze. How much ATP will be produced, per glucose molecule, if the reducing equivalents from NADH produced during glycolysis were transferred to the electron transport chain via the glycerol-3 phosphate shuttle?

Explanation / Answer

Citrate<=> cis-Aconitate<=> Isocitrate

minus H2O. plus H2O

CH2--COO- CH2--COO-

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C---COO- H--- C---COO-

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CH--COO- H--C---COO-

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OH

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