not sure how to answer # 3 and 4 . please help with calculations You will create
ID: 1022517 • Letter: N
Question
not sure how to answer # 3 and 4 . please help with calculations
You will create a series of 2-fold (1/2) dilutions with methylene blue dye in DI water. The first tube will be filled with your stock solution (Considered to have a concentration of 100% or a dilution of 1). Obtain and properly label 6 test tubes. Write the tube number, as well as the expected final concentration (in %) of the solution in that tube. Fill tube 1 with 4.6 ml of Stock Methylene Blue. Fill tubes 2-6 with_ml of DI water. Transfer_ml from Tube 1 to Tube 2.Explanation / Answer
The question asks to make 1/2 dilution solutions in the tubes
So to answer question 3
Since you have added 4.6 mL in tube 1
In tube 2 you can add 2.3 mL of DI water
Then add 2.3 mL of Methylene Blue Dye in the tube this will give you half dilution compared to Tube 1
Question 4 answer is transfer 2.3 mL of Methylene Blue Dye from tube 1
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