When streaking a plate, the lid should be ____ a. Placed on the bench top, top s

Question

When streaking a plate, the lid should be ____ a. Placed on the bench top, top side up b. Placed on the bench top, inside surface up c. Should be held open at an angle to prevent contamination from the air. d. None of the answer choices are correct. e. All of the answer choices are correct. When streaking a plate, the inoculating loop ___ a. Should be placed on the bench top b. Should be heated until it turns bright red c. Should be blown cool with your mouth d. None of the answer choices are correct e. All of the answer choices are correct. Since most bacterial cells have a ___ charge, stains with a ___ charge should be used to stain them. a. Positive/positive b. Positive/negative c. Negative/positive d. Negative/negative e. All of the answer choices are correct. This staining technique is useful for visualizing the glycocalyx of certain bacterial species. a. Negative staining b. Simple staining c. Capsule staining d. Endospore staining. e. Gram staining The negative staining technique utilizes ____ a. Basic fuchsin b. India ink c. Nigrosin d. both (b) and (C) are correct. e. both (a) and (C) are correct. Which of the following is an anaerobe that produces endospores? a. Bacillus b. Mycobacterium c. Clostridium d. a and b e. a and c Endospores are resistant to all of the following EXCEPT: a. Heat b. Radiation c. Acids d. Disinfectants e. Endospores are resistant to all of the above

Explanation / Answer

ans 14. plate should be kept inverted after streaking so that colonies shold not mix together so ans is d where none choice is corret.

15. b becuase loop should be red hot to avoid contamination.

16. bacyerial cell habe negative charge due to presence of teichoic acid hence they stain with positive charge so answer is c

17. to visualise glycocalyx we use negative stain since glycocalx is uncharged.

18. negative stain utilises india ink. so b.

19. ans is e, both bacillus and clostridium form endospore in unfavourable condition.

20. e, endospore is highly resistant to radiation, heat, acid, etc in short every thing.

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