determine the primary structure A small polypeptide fragment of the CFTR protein

Question

determine the primary structure

A small polypeptide fragment of the CFTR protein from chimpanzee was analyzed to determine the primary structure. The following results were obtained: Trypsin digestion and sequencing led to the following 10 sequences: K K K R T-K Q-S-F-K F-S-I-V-Q-K Q-T-G-E-F-G-E-K N-S-I-L-N-P-I-N-S-I-R T-P-L-Q-M-N-G-I-E-E-D Chymotrypsin digestion and sequencing led to the following 4 sequences: K-Q-T-G-E-F T-K-K-Q-S-F S-I-V-Q-K-T-P-L-Q-M-N-G-I-E-E-D G-E-K-R-K-N-S-I-L-N-P-I-N-S-I-R-K-F Endopeptidase V8 (cuts peptide bonds on the carboxyl side of aspartate and glutamate residues) digestion and sequencing led to the following 5 sequences: E D F-G-E T-K-K-Q-S-F-K-Q-T-G-E K-R-K-N-S-I-L-N-P-I-N-S-I-R-K-F-S-I-V-Q-K-T-P-L-Q-M-N-G-I-E

Explanation / Answer

the primary structure is

T-K-K-Q-S-F-K-Q-T-G-E-F-G-E-K-R-K-N-S-I-L-N-P-I-N-S-I-R-K-F-S-I-V-Q-K-T-P-L-Q-M-N-G-I-E-E-D.

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