2. Tomato paste contains lycopene and ?-carotene. A student designed a column ch

Question

2. Tomato paste contains lycopene and ?-carotene. A student designed a column chromatography experiment in which he used alumina for stationary phase. He started by eluting the first band with hexanes and then switched to a mixture of 10% acetone in hexanes. Perform a literature search and determine which was the first band and which was the second one. Give the structure of both compounds and explain your answer. (4 points). Why were you not able to separate as many bands as you did in TLC even though you used the same mobile phase composition? (2 points) 3.

Explanation / Answer

2. Column chromatography of carotene and lycopene. Elution with non-polar hexane gave carotene as the less polar component out of the column first. When switched to 10% acetone, elution of lycopene (more polar than carotene) from the column occurs.

3. the amount of substance loaded on the column is much greater than used for running tlc and therefore we did not obtain as many bands as we saw on tlc from the column.

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