The components from the other page are Lysine, Arginine, the dipeptide composed

Question

The components from the other page are Lysine, Arginine, the dipeptide composed of arginine and threonine, the tripeptide glutathione, and the polypeptide GLU-GLY-MET-LEU

electrophoresis experiment. An illustration of the gel after the run is seen below. Based position of the spots in lanes 1 through 7 (forget about lane S for now) and your expanded drawings of substances 3A through 3E, in which every amino group is protonated and every carboxyl group is deprotonated, determine which spots in the gel are associated with each of those five substances. Enter your answers in the spaces provided below. Assume that peptide bonds were broken in the electrophoresis experiment. 4. DiTerent mixtures of substances 3A through JE (from the previous page) wre run i otherio on the none of the LANE 8. LANE 7 LANE 61 LANE5 LANE 4 LANE 3 LANE 1 You should be able to identify each of the spots at right as being one of the five structures (3A through 3E), on the previous page

Explanation / Answer

Gel electrophoresis

Positive ions move towards -ve electrode and negative ions move towards +ve electrode

Lane 2, Lane 5 and Lane 7 : all amino acid is protonated

Lane 3 and Lane 6 : all amino acid is deprotonated

Identification labels from top to bottom for given shapes would thus be,

arginine

lysine

dipeptide arginine-threonine

Trtrapeptide glu-gly-met-leu

tripeptide glutathione

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