Obtain a B-2 tablet from the TA. Crush the tablet using a mortar and pestle, and
ID: 1026968 • Letter: O
Question
Obtain a B-2 tablet from the TA. Crush the tablet using a mortar and pestle, and weigh 10 mg of the powder. Add the weighted sample to a 1000 mL volumetric flask and dilute to the mark with room temperature, deionized water; this produces a 10 ppm solution of the unknown. Finally, deliver 1.00 mL of the 10 ppm solution to a 250 mL volumetric flask and dilute to the mark with room temperature, deionized water. Calculate and record the ppb of riboflavin in the final solution of the unknown. Finally, using a disposable pipette, pipette you vitamin tablet solution into a fluorescence cute. 0.01182 tabletExplanation / Answer
Answer:
The concentration of diluted soution can be caulucated using the below equation;
C1V1 =C2V2
Where C1 = Initial Concentartion = 10 ppm
V1 = Initial Volume = 1 mL
C2 = FInal Concentartion = ????
V2 = Final Volume = 250 mL
Therefeor according to the equation, 1*10 = 250*C2
C2 = 10/250 =0.04 ppm
Interms of ppb = 0.04 * 1000 {where 1 ppm = 1000 ppb}
Therefore the final concentration = 40 ppb
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