Tris is a common buffer for studying biological systems. Tris is a common buffer

ID: 1031844 • Letter: T

Question

Tris is a common buffer for studying biological systems. Tris is a common buffer for studying biological systems. value 1.00 points 1 out of 3 attempts Be sure to answer all parts. Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems (Ka-5.01 × 10-9 and pKa-8.3) (a) Calculate the pH of the Tris buffer after mixing 18.5 mL of 0.10 M HCI solution with 25.0 mL of 0.10M Tris (b) This buffer was used to study an enzyme catalyzed reaction. As a result of the reaction, 0.00013 mol of H' was produced. What is the pH of the buffer at the end of the reaction? (c) What would be the final pH if no buffer were present?

Explanation / Answer

Solution-

a)

mmol of TRIS --> 25*0.1 = 2.5

mmol of acid = 0.1*18.5 = 1.85

then,

Now the mmol fo TRIS base left --> 2.5-1.85 = 0.65

mmol of TRIS acid formed = 1.85

pH = pKa + log(TRIS/TRIS acid)

pH = 8.3 + log(0.65/1.85)

pH = 7.84

b)

mmol of H+ = MV = 0.00013 = 0.00013 *1000 = 0.13

mmol fo TRIS base left = 2.5-1.85= 0.65

mmol of TRIS acid formed = 1.85 + 0.13 = 1.98

pH = pKa + log(TRIS/TRIS acid)

pH = 8.3 + log(0.65/1.98)

ph = 7.82

c)

pH = -log[H+] ) = -log(0.00013) = 3.88

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Tris (hydroxymethyl) aminomethane, commonly known as Tris, is a weak base that i

Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used a

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Tris is a common buffer for studying biological systems. Tris is a common buffer

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