Capillary electrophoresis offers better resolution than slab-gel electrophoresis

Question

Capillary electrophoresis offers better resolution than slab-gel electrophoresis

because:

a. Detection of the analytes can be made “on column” using detectors either assaying

“along” the column or utilizing total internal reflection to maximize the pathlength, d, in Beer’s

Law, A=ecd.

b. The choice of either electrokinetic detection or pressure injection offers ggreater flexibility

in loading the capillary column.

c. The number of theoretical plates is greater at the higher voltages possible with capillary

electrophoresis.

d. Since the sample sizes are so much smaller, lower voltages can be used with capillary

electrophoresis, so there is less thermal decomposition of the analyte.

e. Only gels can be used with slab electrophoresis, so derivatization of the stationary phase

is not possible.

Explanation / Answer

In slab gel electrophoresis the loading of DNA sample must be very careful while in capillary elecrophoresis either elecrokinetic detection or pressure injection offers greater flexibility in loading capillary column.So b is correct because in this at high voltages this phenomenon occur

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