SHOW WORK/EXPLAIN ANSWER, not just number or word alone. 1.) How many actual QH2
ID: 1034328 • Letter: S
Question
SHOW WORK/EXPLAIN ANSWER, not just number or word alone.1.) How many actual QH2 molecules, total (rather than net) are oxidized in the process of reducing 2 molecules of cytochrome c?
2.) How many cytochrome c molecules are needed to fully reduce 1 molecule of O2?
3.) Oligomycin is an antibiotic that blocks proton transfer through the F0 proton channel of ATP synthase. What is the effect on: a) ATP synthesis?
b) election transport?
c) oxygen consumption when oligomycin is added to a suspension of respiring mitochondria?
d) when dinitrophenol is added to the suspension in (c) above?
4.) A man presents in the emergency room after ingesting insecticide. The Poison Control Center indicates that the insecticide contains cyanide (CN-), which binds to and completely inhibits cytochrome c oxidase. Would ATP synthesis in this mans mitochondria be stopped?
5.) The compound bongkrekic acid binds tightly to the ADP/ATP translocase, stopping it from working. What does this do to: a) electron transport?
b) ATP synthesis?
SHOW WORK/EXPLAIN ANSWER, not just number or word alone.
1.) How many actual QH2 molecules, total (rather than net) are oxidized in the process of reducing 2 molecules of cytochrome c?
2.) How many cytochrome c molecules are needed to fully reduce 1 molecule of O2?
3.) Oligomycin is an antibiotic that blocks proton transfer through the F0 proton channel of ATP synthase. What is the effect on: a) ATP synthesis?
b) election transport?
c) oxygen consumption when oligomycin is added to a suspension of respiring mitochondria?
d) when dinitrophenol is added to the suspension in (c) above?
4.) A man presents in the emergency room after ingesting insecticide. The Poison Control Center indicates that the insecticide contains cyanide (CN-), which binds to and completely inhibits cytochrome c oxidase. Would ATP synthesis in this mans mitochondria be stopped?
5.) The compound bongkrekic acid binds tightly to the ADP/ATP translocase, stopping it from working. What does this do to: a) electron transport?
b) ATP synthesis?
SHOW WORK/EXPLAIN ANSWER, not just number or word alone.
2.) How many cytochrome c molecules are needed to fully reduce 1 molecule of O2?
3.) Oligomycin is an antibiotic that blocks proton transfer through the F0 proton channel of ATP synthase. What is the effect on: a) ATP synthesis?
b) election transport?
c) oxygen consumption when oligomycin is added to a suspension of respiring mitochondria?
d) when dinitrophenol is added to the suspension in (c) above?
4.) A man presents in the emergency room after ingesting insecticide. The Poison Control Center indicates that the insecticide contains cyanide (CN-), which binds to and completely inhibits cytochrome c oxidase. Would ATP synthesis in this mans mitochondria be stopped?
5.) The compound bongkrekic acid binds tightly to the ADP/ATP translocase, stopping it from working. What does this do to: a) electron transport?
b) ATP synthesis?
Explanation / Answer
2 QH2 molecules are oxidized in reducing 1 molecule of cytochrome C. So, for 2 molecules of cytochrome C, 4 QH2 molecules are required to oxidized. 2 cytochromes C molecules are needed to fully reduced 1 molecule of O2. a) By blocking Fo proton channel of ATP synthase, the flow of proton from inter mitochondrial space to mitochondrial matrix will block leading to hyperpolarization of inner mitochondrial membrane, resulting into the obstruction of ATP synthesis. b) Oligomycin blocks ATP synthesis which will significantly reduces the dissipation of protons from inner mitochondrial membrance to matrix, eventually inner mitochondrial membrane develops a large positive charge that the electron transport chain can no longer pump the protons against the gradient. c) Consumption of oxygen is stopped because of inhibation of ATP sysnthesis by olygomycin which is coupled to the activiy of electron transport channel. Electrons released from NADH & FADH2 are eventually transferred through electron transport chain to O2, forming H2O. d) When dinitrophenol is added to the above suspension, it will uncouple the oxidation from phosphorylation. Dinitrophenol will allow the oxidation of NADH & FADH2 and reduction of O2 to H2O but do not permit the ATP synthesis. Yes, cyanide will block ATP synthesis because it will inhibit cytochrome C oxidase which prevents binding of O2 to the final molecule in electron transport chain. In the absence of O2 the electron transport chain will block and hence no ATP is generated. a) If ATP synthesis does not occurs, the transport of electron through electron transport chain will also blocks. As oxidation is coupled with phosphorylation in mitochondrial respiration process. b) If ADP-ATP translocase is blocked, then in absence of ADP the process of ATP formation is inhibited. As ADP moves into the matrix and the newly synthesize ATP is transported out into the cytoplasm.
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