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If a sample of glucose labeled with 14C at carbon-1 (C1) and carbon-6 (C6) was u

ID: 1037165 • Letter: I

Question

If a sample of glucose labeled with 14C at carbon-1 (C1) and carbon-6 (C6) was used as the sole glucose source for glycolysis, at what carbon: aldehyde (-CHO), hydroxymethylene (>CHOH), or methyl-phosphate (-CH2OPO32?) of the intermediate glyceraldehyde 3-phosphate would the label appear?

the aldehyde carbon of all the glyceraldehyde 3-phosphate molecules

The label would not appear in any of the glyceraldehyde 3-phosphate molecules

the methyl-phosphate carbon of all the glyceraldehyde 3-phosphate molecules

Explanation / Answer

the methyl-phosphate carbon of all the glyceraldehyde 3-phosphate molecules when fructose 1,6 biphosphate get transformed to glyceraldehyde-3-phosphate and dihydroxy acetone phosphate. But dihydroxy acetone phosphate get isomerized to glyceraldehyde 3-phosphate so ultimately methyl-phosphate carbon of all glyceraldehyde 3-phosphate will be labeled with C14.

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