an oligomeric protein has a molecular weight of 397000 an oligomeric protein has

Question

an oligomeric protein has a molecular weight of 397000 an oligomeric protein has a molecular weight of 397000 an oligomeric protein has a molecular weight of 397000 An oligomeric protein has a molecular weight of 397000. A 470 mg sample was treated with an excessof 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCI. The product of the complete hydrolysis contained 0.75 mg of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the a-amino groups of other amino acids could be found. NO2 How many polypeptide chains are in this protein? (Enter your answer as a whole number). -NH- -COOH H2 Number 178 CH Tools x 10

Explanation / Answer

mmoles of 2,4-dinitrophenyl derivative = mass / molar mass

                                                          = 0.75 / 256

                                                           = 2.93 x 10^-3 mmol

mmoles of protein = 470 / 397000 = 1.184 x 10^-3

number of poly peptides bonds = 2.93 x 10^-3 / 1.184 x 10^-3

                                                  = 2.5

number of poly peptides bonds = 2

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