thanks bluelirba \"Mandin\" is newly discovered protein. HIPI is a transmembrane

Question

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bluelirba

"Mandin" is newly discovered protein. HIPI is a transmembrane protein of unknown function. GM130 is a known Golgi-resident protein Calnexin is a known ER resident protein. Hela cells were transfected with GFP-Mandin and then treated with either control (top) or HIPI (bottom) siRNAs. After 24 hours, cells were fixed and stained with anti-GM130 (top) or anti-Calnexin (bottom) antibodies and a secondary antibody with a red fluorescent tag. Cells were examined with fluorescence microscopy.

Explanation / Answer

Essentially, since there is co-localization (signified by yellow color => red and green at the same spot) in the third panel in second row, then mandin and calnexin have essentially then same location. And proteins that have the same location in the endomembrane system generally have similar function. However, this cannot be concluded from just this figure so I would not include it in your answer (the information is relevant if you have other figures). Also, because the third panel shows little co-localization, the conclusion reached from the figure is that Mandin is a resident ER protein.

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