Section Name Properties of Enzymes: Acid Phosphatase Experiment #10 Data & Repor

Question

Section Name Properties of Enzymes: Acid Phosphatase Experiment #10 Data & Report Sheet Record absorbance at 410 nm of each solution after adding to 3.0 mL of 0.10 M sodium hydroxide. Absorbance @ 410 nm Tube Conditions On ice, 0°C 2 Room Temperature, 22 C Body Temperature, 37°C, with substrate 4 Body Temperature, 37 C, No Substrate Boiled Enzyme, Incubated at 37'C, with Substrate Comparing tubes 3 and 4, what conclusion can you make about the need for substrate in A-1. order to demonstrate enzyme activity? A-2. Explain why tube 5 would have lower activity than tube 3. A-3. Explain the relative absorbance for tubes 1, 2 and 3. What accounts for the differences? ence of the Reaction on Enzyme Concentration and Fluoride Inhibition Tube Conditions Absorbance @ 410 nm 0 No Enzyme Added 5 5 Drops of Enzyme Solution Added 10 10 Drops of Enzyme Solution Added F 5 Drops of Enzyme Solution Added, with Fluoride Inhibitor 85

Explanation / Answer

A-1: Enzyme binds to the substrate to form the activalted complex. Without the substrate no enzyme activity will be found.

A-2. When you boil the enzyme, the enzyme molecule is denatured and t loses its active sites. The enzyme's activity is regulated by the active sites in it. As the boiled enzyme has less active sites than the normal enzyme, test tube 5 would have lower activity than 3.

A-3.

As the temperature of the system increases, the reaction becomes faster and more of the activated complex (between substrate and enzyme ) forms and the recorded absorbance also increases.

Test tube 3 has the highest absorbance and test tube 1 has the lowest absorbance.

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