Using the information in the table below, enter the correct pH that should be us
ID: 1045676 • Letter: U
Question
Using the information in the table below, enter the correct pH that should be used to separate the following three amino acids by electrophoresis (a) Ala, His, and Arg (b) Lys, Arg, and Glu pKa Values for Ionizable Groups of Amino Acids pKa of ?-NH3+ 9.87 9.04 8.80 9.82 10.25 9.47 9.13 9.78 9.18 9.76 9.74 8.95 9.21 9.24 10.60 9.15 9.10 9.39 9.11 9.72 pKa of Side Chain Amino Acid pKa of ?-COOH Alanine Arginine Asparagine 2.02 Aspartic acid2.10 Cysteine Glutamic acid 2.10 Glutamine Glycine Histidine Isoleucine Leucine Lysine Methionine 2.28 Phenylalanine 2.58 Proline Serine Threonine Tryptophan 2.38 Tvrosine Valine Isoelectric Point (pl) 6.11 10.76 5.41 2.98 5.02 3.08 5.65 6.06 7.64 6.04 6.04 9.74 5.74 5.91 6.30 5.68 5.60 5.88 5.63 6.00 2.35 2.01 12.48 3.86 8.00 4.07 2.05 2.17 2.35 1.77 2.32 2.33 2.18 6.10 10.53 2.00 2.21 2.09 2.20 10.07 2.29Explanation / Answer
At pH 7.64 arginine remain at it's origination point and alanine carry negative charge(COO- group) thus move towards anode and arginine having pI= 10.76 carry positive charge thus it move towards cathode.And these three can be separated.
Lysine at pH 10 exist as negatively charged species and move toward anode and glutamine also exist as negatively charged species but lysine move faster then glutamine and thus can be separated first and arginine remain in solution and seoasepar in last.
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