Using the accompanying table, determine which protein would stick to a carboxyme

Question

Using the accompanying table, determine which protein would stick to a carboxymethyl cellulose ion exchange column (the column has stationary carboxyl groups) at pH 7.0.

Protein                                                Isoelectric Point

Amyloglucosidase

Glucose oxidase

Trypsin inhibitor

-Lactoglobulin

Carboanhydrase

Myoglobin horse

Lentil lectin

Ribonuclease A

Cytochrome C

pI 3.5

pI 4.2

pI 4.5

pI 5.15/5.3

pI 6.0

pI 7.35

pI 7.75/8.0/8.3

pI 9.45

pI 10.65

Amyloglucosidase

Glucose oxidase

Trypsin inhibitor

-Lactoglobulin

Carboanhydrase

Myoglobin horse

Lentil lectin

Ribonuclease A

Cytochrome C

pI 3.5

pI 4.2

pI 4.5

pI 5.15/5.3

pI 6.0

pI 7.35

pI 7.75/8.0/8.3

pI 9.45

pI 10.65

Explanation / Answer

SOLUTION:

Ribonuclease is a protein which would stick to a carboxymethyl cellulose ion exchange column (the column has stationary carboxyl groups) at pH 7.0.

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