FIND ESTIMATED AND CALCULATED KM ND VMAX VALUES for ALL 3 test! Thank YOU Part 1

Question

FIND ESTIMATED AND CALCULATED KM ND VMAX VALUES for ALL 3 test! Thank YOU

Part 1 Michaelis-Menten Plot Reaction Rate (mmol/min) 0.0425 0.0625 0.115 0.0925 0.0975 L-DOPA Concentration (mM) [S] 1.5 03 Part 2 0.0225 0.0325 0.04 0.0525 0.11 1.5 Part 3 0.0275 0.07 0.0235 0.0375 0.0425 Lineweavwer-Burk Lineweaver- Burk Data Test set 1-Tyrosinase 1/rate ((mmol/min)-1) 24 16 Test set 2-Thiourea 1/rate Test set 3-Benzoic Acid rate ((mmol min)-1 36 14 42 27 24 R- 092 0.67 0.5 0.25 0.13 31 25 19 Tyrosinase irear(rosnase Lewar (h

Explanation / Answer

The equation for the Lineweaver-Burk plot of 1/v versus 1/[S] can be written as follows.

1/v = KM/vmax * 1/[S] + 1/vmax .............. Equation 1

Where S is the substrate, KM is the Michaelis-Menten constant and vmax is the maximum rate observed.

Part 1: Using Tyrosinase enzyme:

The equation of Lineweaver-Burk plot is given as 1/v = 15.84345 * 1/[L-DOPA] + 5.91984

By comparing the above equation with equation 1, you can get

1/vmax = 5.91984, i.e. vmax = 0.1689 mmol/min

KM/vmax = 15.84345

i.e. KM*5.91984 = 15.84345

i.e.  KM = 2.676 mM

Part 2: Using Thiourea enzyme:

The equation of Lineweaver-Burk plot is given as 1/v = 37.4843 * 1/[L-DOPA] + 6.48301

By comparing the above equation with equation 1, you can get

1/vmax = 6.48301, i.e. vmax = 0.1542 mmol/min

KM/vmax = 37.4843

i.e. KM*6.48301 = 37.4843

i.e.  KM = 5.782 mM

Part 3: Using Benzoic acid enzyme:

The equation of Lineweaver-Burk plot is given as 1/v = 6.94851 * 1/[L-DOPA] + 25.05626

By comparing the above equation with equation 1, you can get

1/vmax = 25.05626, i.e. vmax = 0.0399 mmol/min

KM/vmax = 6.94851

i.e. KM*25.05626 = 6.94851

i.e.  KM = 0.277 mM

Related Questions

Navigate

• Browse (All)
• Browse F
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.