Chromatographic Determination of Sugars (Sucrose, Glucose, Fructose) Study Quest

Question

Chromatographic Determination of Sugars (Sucrose, Glucose, Fructose)

Study Questions 1. The column should always be handled with care. Discuss what would happen if the stationary phase is compromised 2. Why is it important to keep the flowrate constant? 3. Predict the elution time if the temperature is slightly hotter? 4. Bubbles must not be present in the syringe. Will this air pockets cause positive or negative error? Explain why. 5. Rationalize the order of clution of the different sugars used

Explanation / Answer

1. Retention time would be shortened, hence not giving the compound enough time to be analyzed.
2. To keep the pressure-drop in check and to minimize all the other effects caused due to irregular or high velocities.
3. At slightly hotter temperatures, the separation process becomes faster hence the elution time shortens.
4. It will cause a negative error, the refractive index of solute tends to be lesser than that of mobile phase
5. The order of elution : Fructose, glucose, sucrose

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