biochem Polyacrylamide is: a) af fluorescent probe b) a tagging agent use for NM
ID: 1047616 • Letter: B
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biochem Polyacrylamide is: a) af fluorescent probe b) a tagging agent use for NMR c) a material used to make protein electrophoresis gels d) used to bind antibodies in Western blotting The reagent used in the Edman sequencing method is: a) phenylistocnate b) phenylisothiocyanate c) mercaptoethanol d) an antibody specific to the protein you are analyzing Ultracentrifugation is used for: a) purifying proteins b) determining protein sequence c) determining protein conformation d) determining molar mass of proteins To determine the location of proteins in cells, perhaps the best method is: a) NMR b) mass spectroscopy c) SDS-PAGE d) fluorescence microscopy NOESY is a special application of which method? A) electrophoresis b) chromatography c) NMR d) mass spectrometry MALDI-TOF is a special application of which method? A) electrophoresis b) chromatography c) NMR d) mass spectrometry Complete 3-dimensional images of proteins may be obtained using which method(s)? A) mass spectrometry b) NMR c) x-ray crystallography d)b and c Complete 3-dimensional images of proteins in solution may be obtained using which method(s)? A) mass spectrometry b) NMR c) x-ray crystallography d)b and c Complete 3-dimensional images of proteins in the solid state may be obtained using which method(s)? A) mass spectrometry b) NMR c) x-ray crystallography d)b and c The fluorimetric/spectrophotometric method which uses antibodies is called: a) MAIDI-TOF b) NOESY c) Western blotting d) ELISA The protein pyruvate decarboxylase catalyzes the following reaction: Pyruvate + coenzyme A = acetyl Co- A + CO_2 The molecules biotin and lipoic acid are needed for pyruvate decarboxylase to function properly. List each of these substances as enzyme (E), substrate (S), product IP) or cofactor .Explanation / Answer
1) Polyacrelamide is c) a material used to make protien electrophoresis gel. When voltage is applied the smallar protiens moves faster than larger one in the gelof acrelamide.
2) Reagent used for Edman sequancing method is b) Phenylisothiocyanate. This reagent reacts with amino group of terminal amino acid & which is selectively seperated from amino acid.
3) Ultracentrifugation is used for d) determination of Molar mass of protiens. Molar mass is determined without using molar mass standerds or interferences in sieving matrix.
4) To determine location of protiens in cell d) Fluoroscence microscopy is used. This can be done by using reporter protiens or fluorescent dyes.
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