Complete in advance and show to your instructor at the beginning of class. You w

Question

Complete in advance and show to your instructor at the beginning of class. You will be using these calculations in the laboratory procedure. To dilute a protein sample 40-fold, how many mL of your protein sample must be used to make a final volume of 4 mL of the diluted volume? How many mL of water will you need to add to make the 4 mL volume? You will be using this dilution scheme to construct dilutions of EW & S1 for BC.A protein analysis Perform a similar procedure to above to calculate how to make 4 mL of a 5-fold dilution How many mL of protein sample must be used? How many mL of water? You will be using this dilution scheme to construct dilutions of S2 & E3 for BCA protein analysis You do NOT need to perform a dilution of E4. Use the undiluted sample for your BCA protein analysis (This is technically considered a "1-folcf" dilution)

Explanation / Answer

(1)

DF = Vf / Vi

Where, Vf = final volume , Vi = initial volume , DF = degree of fold

=> Vi = Vf / DF

=> Vi = 4 / 40 = 0.1 mL

0.1 mL of protein has to be diluted.

Volume of water = 4 mL - 0.1 mL = 3.9 mL

(2)

Vi = Vf / DF

Vi = 4 / 5 = 0.8 mL

Volume of protein to be diluted = 0.8 mL

Volume of water needed = 4 - 0.8 = 3.2 mL

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