the enzywies wl bindl up ul the analog ins kod of the nornal sub shate it coumpe
ID: 1047825 • Letter: T
Question
the enzywies wl bindl up ul the analog ins kod of the nornal sub shate it coumpek wf the noomal oul Transition-State Analogs -Competitive Inhibitors and Catalytic Antibodies- Transition Snpekc Transition-state analogs (TSAs) are stable compounds whose structures resemble unstable transition states. Transition state analogs can have application as therapeutics as either competitive inhibitors or in the generation of catalytic antibodies. Competitive Inhibitors TSAs can bind the active siteof a target enzyme active site with high affinity. preventing substrate binding (Competitive Inhibition). . o NO Catalytic Antibodies (Abzymes) Substrate Antibodies generated against a TSA may also bind the substrate, catalyzing 0-(H) the reaction by promoting formation of the transition state Transition State o ny unstable 0 cn, no, presence NO, ex-(CH,1 -0-w.(.)-o-o@ CH,,- + HA NO2 Transition State Analog Products crboylae p-NaroanilineExplanation / Answer
In enzyme-catalyzed reactions, enzymes function by lowering activation energy and that incredible catalytic rate enhancements caused by enzyme is governed by tight binding to the unstable transition state structure. Transition state analogs mimic this high energy intermediate but do not undergo a catalyzed chemical reaction and can therefore bind much stronger to an enzyme than simple substrate or product analogs.
Catalytic antibodies bind very tightly to the transition-state analogs that were used to produce them during the immunization process. The transition-state analogs only bind and do not react with catalytic antibodies. For this reason, transition-state analog can interfere with the catalytic reaction by binding in the antibody binding pocket, thereby preventing any substrate molecules from binding and reacting. This inhibition by the transition-state analog is always observed with catalytic antibodies, and is used as a first level of proof that catalytic antibodies are responsible for any observed catalytic reaction.
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