In TLC, why do we dry the spots after we inject the samples on the plate? In TLC
ID: 1048121 • Letter: I
Question
In TLC, why do we dry the spots after we inject the samples on the plate?In TLC why is important to avoid touching the spots with mobile phase while putting the plate in the chamber?
Assume you have a mixture of two aminoacids and run TLC to identify them but spots overlapped at the end of the experiment. How can you overcome this problem? (What should you do to separate these overlapping spots?)
In TLC, why do we dry the spots after we inject the samples on the plate? In TLC, why do we dry the spots after we inject the samples on the plate?
In TLC why is important to avoid touching the spots with mobile phase while putting the plate in the chamber?
Assume you have a mixture of two aminoacids and run TLC to identify them but spots overlapped at the end of the experiment. How can you overcome this problem? (What should you do to separate these overlapping spots?)
Explanation / Answer
After injecting the samplea on TLC they are dried because if they are not dried then solvent remianing on the spot will give tailing on TLC run. (Uneven run of spot)
The spotson TLC shpuld not touch mobile phase because if they come in touch with mobile phase before TLC is run then the spots will diasolve away in mobile phase and there will be no separation.
When two spots of amino acids are overlapped on TLC then mobile phase composition should be changed. The compoaition of mobile phase should have been more on non polar side. The non polar component should be more compared to polar component. When running the TLC in these mobile phase the amino acid with nonpolar property will go further up on TLC and polar amino acid will stay at lower level on TLC.
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