Identify the stationary and mobile phases of the TLC from this experiment. Expla

Question

Identify the stationary and mobile phases of the TLC from this experiment. Explain the resulting trend of Rf values for the various components of the spinach extract based on the principles of thin-layer chromatography. Address both the stationary and mobile phases. What are two common methods used to visualize colorless compounds on a TLC plate based on the reading? TLC can be used in six applications of organic chemistry. Which two applications were used in this experiment? Justify your answer.

Explanation / Answer

1.

Stationary phase is Silica gel coted aluminum plate

or we can call it as SiO2

Mobile phase is solvent mixture which you used for run the plate

2.

you should mention the structure of the bases

one this is more polar compound will have less Rf and less polar compound will have more Rf

3.

KMnO4 charing agents for alcohol identification

2,4DNP solution for carbonyl identification

4.

to identify the reaction is proceeding or not

it is very samll scale we can even separate the closed spots

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