A reversed phase HPLC analysis follows a gradient elution scheme, starting with

Question

A reversed phase HPLC analysis follows a gradient elution scheme, starting with a 10:90 methanol:water solution, and ending with a 60:40 methanol:water solution. This is done because the 60:40 solution is polar than the 10:90 solution, which helps to elute the more compounds. more, polar more, nonpolar less, polar less, nonpolar Two organic molecules were separated on a 150 mm reversed phase column with a flow rate of 1.0 mL min^-1. Compound 1 had a retention time of 3.5 min and a width of 0.35 min and compound 2 had a retention of 4.0 min and a width of 0.45 min. What is the resolution? 0.80 1.2 5.0 9.4

Explanation / Answer

The correct answer is d. less, non polar

The 60:40 solution of methanol and water is less polar because the polarity of methanol is less than water .

Organic compounds are non-polar thus this mixture will helps to elute more non-polar compounds.

Water has a polarity of 10.2                 
Methanol has a polarity of 5.1

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