A glucose solution has a concentration of 1 g/dL. One mL of this glucose solutio

Question

A glucose solution has a concentration of 1 g/dL. One mL of this glucose solution is diluted with water to a final volume of 10 Ml in a volumetric flask. Then, 1 mL of the diluted glucose solution is transferred to a test tube and 4 mL water is added. The solution in the test tube is mixed well. What is the final concentration (in mg/dL) of the glucose solution in the test tube? Show all calculations. The actual concentration of your solution as determined from a standard curve graph is 19.5 mg/dL. What is the percent error of your solution? Explain why we use a reagent blank to set the spectrophotometer to 0% a instead of just the water blank. Under what circumstances would it be necessary to use a sample blank? Define the following and explain their importance in spectrophotometer: Complementary color lambda max

Explanation / Answer

Q1.

C1V1 = C2V2

C2 = V1/V2*C1 = 1/10*1 = 0.1 g/mL

then...

we take

1 ml

V3*C3 = V4*C4

C4 = 1*0.1 / (1+4)

C4 = 0.02 g/mL

final oncnetration is 0.02 g/mL

b)

0.02 g --> 0.02*10^3 = 20 mg/mL

1 dL = 100 mL

20 mg / mL * 100 mL / dL = 2000 mg/ mL

error = (2000 -20)/(20) * 100 = 9900 %

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