Five mutant strains of E. coli require one or more of the supplemented sugars fo

Question

Five mutant strains of E. coli require one or more of the supplemented sugars found in the Glycolysis pathway in order to convert 1,3-P-glycerate to acetyl-CoA. From the data below (where “+” = acetyl-CoA produced, “0” = no acetyl-CoA produced) draw the metabolic pathway including the mutated enzymes (corresponding with the different mutants) that block the pathway.

Mutant   Acetyl-CoA    3-P-glycerate    2-P-glycerate    Phosphoenolpyruvate      Pyruvate
1              +                  0                       0                          0                                   +
2              +                  0                       0                           0                                  0
3              +                  0                       +                           +                                  +
4              +                   +                       +                          +                                   +
5              +                   0                      0                            +                                  +

Explanation / Answer

precursor>>> 3P>>>2P>>>Phosph pyruvate>>>pyruvate>>>ACoA the mutants go on the arrows in the order 4 3 5 1 2

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