n a recent publication, gold nanoparticles conjugated to DNA(self-hybridized) ex
ID: 1059202 • Letter: N
Question
n a recent publication, gold nanoparticles conjugated to DNA(self-hybridized) exposed to Radio Frequency (RF) energy resulted in the dehybridization of the DNA as shown in the figures below:
A. Describe the potential structure of the hybridized and dehybridized DNA.
B. Describe how the A260 reading could change under RF.
In a recent publication, gold nanoparticles conjugated to DNA(self-hybridized) exposed to Radio Frequency (RF) energy resulted in the dehybridization of the DNA as shown in the figures below: AA OTA On 0,26 0.25 0.24 0.23 0.22 0.2 0,20 0.19 me (min) A. Describe the potential structure of the hybridized and dehybridized DNA. B. Describe how the A260 reading could change under RF. C. Propose a mathematic equation to model the dehybridization seen in the figure. Chapter 9: Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling DNA sequences within the endogenous genome and their functional outputs are now easily edited or modulated in virtually any organism of choice. A. Describe the mechanisms of how CRISPRICAS9 allows for the editing of a host genome B. Describe an application of CRISPR/CAS9 that is not disease related.Explanation / Answer
Ans.
The DNA nanostructures are promising construction materials to bridge the gap between self-assembly of functional molecules and conventional top-down fabrication methods in nanotechnology. Their positioning onto specific locations of a microstructured substrate is an important task towards this aim. Here we study manipulation and positioning of pristine and of gold nanoparticle-conjugated tubular DNA origami structures using ac dielectrophoresis. The dielectrophoretic behavior was investigated employing fluorescence microscopy. For the pristine origami, a significant dielectrophoretic response was found to take place in the megahertz range, whereas, due to the higher polarizability of the metallic nanoparticles, the nanoparticle/DNA hybrid structures required a lower electrical field strength and frequency for a comparable trapping at the edges of the electrode structure. The nanoparticle conjugation additionally resulted in a remarkable alteration of the DNA structure arrangement. The growth of linear, chain-like structures in between electrodes at applied frequencies in the megahertz range was observed.
A. Secondary structure favours dehybridization. Primary structure favours hybridization. Single strand tend to absorb first on the gold nanoparticles.
B. A260/A280 ratio is studied to observe the purity of the DNA so radio frequency ablation will certainly cause variation in the value of that.
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