Part C High-pressure liquid chromatography is a method used in chemistry and bio

Question

Part C High-pressure liquid chromatography is a method used in chemistry and biochemistry to purity chemical substances. The pressures used in this procedure range from around 500 kilopascals (500,000 Pa to about 60,000 kPa (60.000,000 Pa), Itis often convenient to knowthe pressure in torr. an HPLC procedure is running at a pressure of 1.60x10' Pa, what is its running pressure in ton? Express the pressure numerically in torr. 1.60x107 Pa Submit Hints My Answers Give Up Review Part Incorrect, Try Again a la

Explanation / Answer

B)

V =18 L

n = 0.25 mol of N2

n = 0.25 mol of O2

n = x mol of He

t = 0°C = 273 K

P total = 1 atm

find grams of He

so...

PV = nRT

n = PV/(RT)

n = (1*18)/(0.082*273) = 0.8040 mol of gases

so..

mol of He = 0.8040 - 0.25 -0.25 = 0.554 mol of gas

MW of He = 4 g /mol

so

mass = mol*MW = 0.554*4 = 2.216 g of He

c)

Change

P = 1.6*10^7 Pascal

760 torr = 101325 Pa

sp..

P in torr = 1.6*10^7 Pa * 760 torr / 101325 Pa = 120009.869 torr

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