A mixture of arginine, glutamic acid, and leucine is applied to the center of an

Question

A mixture of arginine, glutamic acid, and leucine is applied to the center of an electrophoresis plate. After the pH is adjusted to 6.0 and an electric current is applied, the components of the mixture separate. Which of the following describes the plate after the experiment has been completed?

A. Leucine migrates towards the negative electrode, arginine migrates towards the positive electrode, and glutamic acid stays near the center.

B. Glutamic acid migrates towards the negative electrode, arginine migrates towards the positive electrode, and leucine stays near the center.

C. Arginine migrates towards the negative electrode, glutamic acid migrates towards the positive electrode, and leucine stays near the center.

D. Leucine migrates towards the negative electrode, glutamic acid migrates towards the positive electrode, and arginine stays near the center.

Explanation / Answer

Ans is C

Arginine has Isoelectric point value of 10.5 at pH 6 and the carboxyle group forms carboxilic acid -COOH and _NH2+,retain 1+ electrone So migrates towards the negative electrode,

glutamic acid has Isoelectric point value 3.2 at 6 pH -COOH renmains with 1 negative electon So migrates towards the positive electrode,

and leucine stays near the center.

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.