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A microbiologist performed a protein extraction from E.coli cells. After the ext

ID: 1077237 • Letter: A

Question

A microbiologist performed a protein extraction from E.coli cells. After the extraction she did her best to separate the various impurities from the protein of interest in the sample. Her UV absorbance measurement gave her an absorbance of 1.493 at 280 nm and 0.143 at 260 nm. What is the approximate concentration in mg/mL) of her protein sample? Question 7 1.5 pts If you were asked to quantify vitamin B12 using UV-VIS, what would be an appropriate wavelength to use for your assay? Note: Report your answer in nm. This data e (also linked on the main page of Canvas) may be useful.

Explanation / Answer

A microbiologist performed a protein extraction from E.coli cells. After the ext

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