This is regarding Vmax, reaction rates, and the presence of a competitive inhibi

Question

This is regarding Vmax, reaction rates, and the presence of a competitive inhibitor in biochemistry.

If an enzyme reaction rate is approaching the Vmax, with a large excess of substrate present, which of the following would have the least effect on the rate of reaction? Add moderate amount of a competitive inhibitor Add a moderate amount of a non-competitive inhibitor Add more enzyme to the mixture Add a moderate amount of an irreversible inhibitor Decrease the Temperature of the Reaction by a moderate amount How can Vmax be approached despite the presence of a competitive inhibitor? Increase enzyme concentration to very high levels Increase substrate concentration to very high levels Increase the temperature of the reaction Decrease the temperature of the reaction Add a non-competitive inhibitor to the reaction mixture

Explanation / Answer

Enzyme catalysis

If a reaction is approaching its Vmax value, while still having large excess of substrate left in the system (that is all the enzyme sites are occupied at this stage), least effect would be observed by the following,

Add moderate amount of competitive inhibitor

This may react with the excess substrate without affecting the reaction

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We may approach Vmax despite presence of a competitive inhibitor as,

Increase substrate concentration to a very high level

This would ensure that sufficient substrate remains available t all times to form the product.

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