(#7) and SDs gel electrophoresis troJ- QUESTIONS Why should there be a small poc

Question

(#7) and SDs gel electrophoresis troJ- QUESTIONS Why should there be a small pocket of air above the solution in the dialysis bag during the dialysis procedure? Why should substances like proteins be lyophilized for storage purposes? Why not just freeze a protein-containing solution and store as it is? 1. 2. 3. Why is a lyophilizing bottle not filled to capacity? 4. Why is the heat of sublimation of water lower than the heat of sublimation of proteins? ud CuD A 7acharias, & R. Y. Tsien. (2000) Biochemistry, mutagenesis, and

Explanation / Answer

Ans 1 :

A small pocket of air above the solution in the dialysis bag must be left during the dialysis procedure , because this air pocket allows the tube to float near the top of the dialysis solution , and also keeps it away from the stirring bar.

So this air pocket is necessary above the dialysis solution.

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