Waste Disposal All of today\'s solutions can go down the sink. Pre-lab lecture W

Question

Waste Disposal All of today's solutions can go down the sink. Pre-lab lecture Write in your notebook all of the molecules and ions that exist in an aqueous pH-4.5, 35 mM acetate buffer made with acetic acid and sodium hydroxide. Label any spectator ions. Calculate acetic acid needed to make a 20 mM solution in 1 L. Note: 35 mM 0.035 M = 0.035 mol/L. 1. Calculate how many moles you need for 1L 2. Calculate how many grams of acetic acid that is. 3. Use the density of the glacial id, 1.05 g/mL, to calculate how many ml. you will need to use. Show your instructor or TA this calc make sure you have the correct answer before going forward 1n Step 2: Experimental Procedure or the rest of In this experiment, you will prepare a dilute buffer that we will luse t semester when doing High Performance Liquid Chromatography (HPLC) 1 of 35 mM Acetate Buffer, pH = 4.5 1 l of distilled water into y

Explanation / Answer

1) 20 mM = 20 * 10^-3 M = 0.020 mole / L

20 mM of 1 L solution of acetic acid = 0.020 * 1 = 0.020 mole.

0.020 moles acetic acid you need for 1 L solution.

2) grams of acetic acid = 0.020 * 60.05 = 1.201 gm

3)

density of glacial acetic acid = 1.05 g /ml

so volume of glacial acetic acid need = mass / density = 1.201 / 1.05 = 1.144 ml.

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