So , my teacher semi-sucks at lecturing and I\'m not a good listener. These are

Question

So , my teacher semi-sucks at lecturing and I'm not a good listener. These are all suppose to be review questions. I'd post them separately but honest I doubt there are many people able to answer these so I'd like for them to be found as a group. Just answer them as you can, I really appreciate any help.

1.What is the “yellow crescent” and what does it tell us about asymmetric cell division?
2.How were the par genes identified? What is the consequence of par gene mutation (on C. elegans development)?
3.How is asymmetric localization of PAR proteins regulated?
4.Antagonisitic interactions between PAR complexes play a conserved role in establishing complementary membrane domains. How does this work?
5.How is symmetry broken in C. elegans? After the “posterior” pole is defined, how is asymmetry generated?
6.What cytoskeletal regulator is provided by sperm? What is the molecular switch that generates the cellular movements driving asymmetry?
7. How does the yeast 2-hybrid assay work? How would you use it to identify interaction partners for a protein of interest?
8. In general, how does the PAR3/6/aPKC complex regulate asymmetric cell division (think: how does the complex connect to cell fate determinants)?
9. How does the PAR6/aPKC complex regulate NUMB localization? How is activity of the PAR6/aPKC complex regulated during interphase? On entry into mitosis?
10. How does Numb work? What happens when you remove it from neural progenitor cells?
11. How about Notch? What is the relationship between Numb and Notch in asymmetric cell division?
12.What are potential points of regulation in the Notch pathway? Think of ways that you could increase or decrease Notch signaling.
13. What is Furin? How is related to Notch? How is it related to our National Defense? 14. Where else does cleavage occur in the Notch pathway? How does proteolysis regulate Notch signaling?
15. What are the cytoskeletal rearrangements involved in neuronal polarization?
16. How are positional signals in the environment (think UNC-6 or the TGF-beta ligand) used to determine the site of axon formation (and thus the axis of polarization)?
17. How are the different PAR proteins regulating neuronal polarity?
18. PI3K is everywhere!?! Why do so many signaling pathways use PI3K?

Explanation / Answer

7. How does the yeast 2-hybrid assay work? How would you use it to identify interaction partners for a protein of interest? A yeast two hybrid is an in-vivo test for protein-protein interactions. By coupling a protein of interest to the gal4 DNA binding domain and then coupling something else (or a cDNA library if you want to survey all possible interactors) to the gal4 activation domain, you can activate the downstream expression of reporter genes (downstream from the gal promoter) upon successful interaction between the two proteins of interest.

Related Questions

Navigate

• Browse (All)
• Browse S
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.