44. Joey is just starting in a lab studying the Na protein that has been assembl
ID: 11376 • Letter: 4
Question
44. Joey is just starting in a lab studying the Na
protein that has been assembled with phospholipids into vesicles. Deciding to impress his labmates, he
decides to do a quick experiment to show that the Na
with part of the lab supply of oubaine, which inhibits this carrier’s function, expecting that the vesicle size
will show a huge change.
A) Do typical animal cells
expand,
B) Now Joey looks at his vesicles, and something must be wrong!…Nothing is happening! His vesicles
have not changed size -
vesicles and was functioning prior to the oubaine treatment.
Explanation / Answer
44. Joey is just starting in a lab studying the Na
+K+ ATPase. He is given a preparation of the purified
protein that has been assembled with phospholipids into vesicles. Deciding to impress his labmates, he
decides to do a quick experiment to show that the Na
+K+ ATPase is functional, and treat your vesicle preps
with part of the lab supply of oubaine, which inhibits this carrier’s function, expecting that the vesicle size
will show a huge change.
A) Do typical animal cells
SHRINK or EXPAND following treatment with oubaine? Circle shrink or expand, and briefly explain why.
It is well understood that the classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrrane sodium pump, especially at the higher concentrations attainable in vitro or intraveneous dosage. Inhibition of the sodium pump and the secondary effect on the handling of calcium ions by sodium calcium exchanger (NCX) is widely believed to underlie the original beneficial effect as an inotropic agent following intravenous use of ouabain;
B) Now Joey looks at his vesicles, and something must be wrong!…Nothing is happening! His vesicles have not changed size - why? Assume that the Na+K+ ATPase was assembled correctly in the vesicles and was functioning prior to the oubaine treatment.
Ouabain binds & enhibits the plasma membrane's sodium pump - that's it's normal function.
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