Here is a piece of a dsDNA helix. 1TACAATATCTCATGTGCCCAGCGGAGGTCCATTCCATCGGTATGG

Question

Here is a piece of a dsDNA helix.

1TACAATATCTCATGTGCCCAGCGGAGGTCCATTCCATCGGTATGGCGCCCGCATTTTAGCTGGACCAGCCC 3

2ATGTTATAGAGTACACGGGTCGCCTCCAGGTAAGGTAGCCATACCGCGGGCGTAAAATCGACCTGGTCGGG 4

We don't know where the 5' and 3' ends are so there are 4 ways to view this:

Option 1:
The promoter could be on the left side of the section, and the number 1 is on the 5' end.
Option 2:
The promoter could be on the left side of the section, and the number 2 is on the 5' end.
Option 3:
The promoter could be on the right side of the section, and the number 3 is on the 5' end.
Option 4:
The promoter could be on the right side of the section, and the number 4 is on the 5' end.

For each option assume the first nucleotide seen is the first nucleotide transcribed into the mRNA.

As a reference, the translated protein for option 4 is:

(N-terminus) Met-Arg-Ala-Pro-Tyr-Arg-Trp-Asn-Gly-Pro-Pro-Leu-Gly-Thr (C-terminus)

**Transcribe and translate each of the other options for this section of DNA.**

Explanation / Answer

Used http://www.vivo.colostate.edu/molkit/translate/index.html to help decode Option 1 (N-terminus) Tyr Asn Ile Ser Cys Ala Gln Arg Arg Ser Ile Pro Ser Val Trp Arg Pro His Phe Ser Trp Thr Ser Pro (C-terminus) Option 2 (N-terminus) Met LEu Ser Thr Arg Val Ala Ser Arg Gly Ser His Thr Ala Gly Val Lys Ser Thr Trp Ser Gly (C-terminus) Option 3 (N-terminus) Gly Leu Val Gln Leu Lys Cys Gly Arg His Thr Asp Gly Met Asp Leu Arg Trp Ala His Glu Ile Leu (C-terminus)

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