4. The linear bacteriophage lambda genomic DNA has at each end a single-strand e

Question

4. The linear bacteriophage lambda genomic DNA has at each end a single-strand extension of 20 bases. (These are “sticky ends” but are not, in this case, produced by restrictions enzyme digestion.) These sticky ends can be ligated to form a circular piece of DNA. In a series of separate tubes, either the linear or circular forms of the DNA are digested to completion with EcoRI, BamHI, or a mixture of the two enzymes. The results are shown here.
a) Which of the samples (A or B) represents the circular form of the DNA molecule?
b) What is the total length of the linear form of the DNA molecule?
c) What is the total length of the circular form of the DNA molecule?
d) Draw a restriction map of the linear form of the DNA molecule. Label all restriction enzyme sites as EcoRI or BamHI.

Explanation / Answer

There is no results attached to your question. I will try my best to find the best links to help you answer the question. I am assuming that you looking at a gel image where the lambda DNA was digested with the restriction enzymes. Links: http://www.apsnet.org/edcenter/K-12/TeachersGuide/PlantBiotechnology/Pages/Activity3.aspx http://www.carolina.com/text/teacherresources/instructions/biotech/dna_restriction_analysis_kit_teachers_manual.pdf http://www.vivo.colostate.edu/hbooks/genetics/biotech/gels/agardna.html http://faculty.plattsburgh.edu/donald.slish/Electrophoresis.html http://people.rit.edu/rhrsbi/GEPages/LabManualPDF5ed/15%20electrophoresis.pdf http://delliss.people.cofc.edu/virtuallabbook/LabReadings/Miniprep/MINIPREPARATION.pdf

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