Test 3 ESOH 280 Microbiology and Public Health 14. is commonly used examine spat

Question

Test 3 ESOH 280 Microbiology and Public Health 14. is commonly used examine spatial interaction of an antigen of interest with its environment 1. ELISA 2. Immunomagnetic separation 3. Fluorescent immunoassay 4. Western blot 15. Northern Blot is used to detect 1. DNA 2. RNA 3. Protein 16. Transcription happens in the 1. nucleus 2. cytoplasm 3. ribosome 4. codon 17. What is the complementary DNA strand to this following sequence: ATC GAT TAG CAT . AGT CAC CTT AGT 2. TAG CTA CTC GTA 3. TAG CTT AAG GTA 4. TAG CTA ATC GTA 18. What is tRNA? 1. Creates ribosomes 2. Transcribed from DNA 3. Transfers specific amino acids 19. What type of cell is used in monoclonal antibody generation? 1. Cardiac 2. Hybridoma 3. Myeloma 4. Hepatocyte 20. True or False. FISH probes are fluorescently-labeled and designed to bind only to non- complementary DNA? 1. Ture 2. False

Explanation / Answer

15)RNA

A northern blot is a laboratory method used to detect specific RNA molecules in a mixture of RNA. Northern blotting can be used to analyze a sample of RNA from a particular tissue or cell type in order to measure the RNA expression of particular genes.

16)Nucleus

It must occur in the nucleus where the DNA in the cell is located.

18)Transfers specific amino acids

transfer RNA is a type of RNA found in cells. It is single stranded and 10–15% of the total RNA. tRNA is synthesized in the nucleus of the DNA. It is also known as Adapter RNA. The major function of the tRNA is to carry amino acids at the time of Protein synthesis

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